Construct: shRNA TRCN0000301004

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004897.3-1746s21c1
DNA Barcode:
CCGAGTGCAGATGTTATTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MINPP1 (9562)
Gene Description:
multiple inositol-polyphosphate phosphatase 1
Transcript:
RefSeq NM_004897.3 (NON-CURRENT)
Match location:
Position 1746 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000301004)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGTGCAGATGTTATTAAA
Hairpin Sequence:
5'-CCGG-CCGAGTGCAGATGTTATTAAA-CTCGAG-TTTAATAACATCTGCACTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGTGCAGATGTTATTAAACTCGAGTTTAATAACATCTGCACTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGTGCAGATGTTATTAAACTCGAGTTTAATAACATCTGCACTCGG-3'

Other clones with same target sequence:

  1. TRCN0000052745