Construct: shRNA TRCN0000301057

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003896.3-1361s21c1
DNA Barcode:
GTGGAGGCATTGATCGTGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ST3GAL5 (8869)
Gene Description:
ST3 beta-galactoside alpha-2,3-sialyltransferase 5
Transcript:
RefSeq NM_003896.3 (NON-CURRENT)
Match location:
Position 1361 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000301057)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGAGGCATTGATCGTGAAT
Hairpin Sequence:
5'-CCGG-GTGGAGGCATTGATCGTGAAT-CTCGAG-ATTCACGATCAATGCCTCCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGAGGCATTGATCGTGAATCTCGAGATTCACGATCAATGCCTCCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGAGGCATTGATCGTGAATCTCGAGATTCACGATCAATGCCTCCAC-3'

Other clones with same target sequence:

  1. TRCN0000036137