Construct: shRNA TRCN0000301175

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009058.2-1382s21c1
DNA Barcode:
GATGCAGAACTATTCAAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ralgds (19730)
Gene Description:
ral guanine nucleotide dissociation stimulator
Transcript:
RefSeq NM_009058.2 (CURRENT)
Match location:
Position 1382 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000301175)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGCAGAACTATTCAAGAAA
Hairpin Sequence:
5'-CCGG-GATGCAGAACTATTCAAGAAA-CTCGAG-TTTCTTGAATAGTTCTGCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGCAGAACTATTCAAGAAACTCGAGTTTCTTGAATAGTTCTGCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGCAGAACTATTCAAGAAACTCGAGTTTCTTGAATAGTTCTGCATC-3'

Other clones with same target sequence:

  1. TRCN0000055075