Construct: shRNA TRCN0000301385

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007434.3-1452s21c1
DNA Barcode:
TCACTTCAGAAGTGGACACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Akt2 (11652)
Gene Description:
thymoma viral proto-oncogene 2
Transcript:
RefSeq NM_007434.3 (NON-CURRENT)
Match location:
Position 1452 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000301385)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCACTTCAGAAGTGGACACAA
Hairpin Sequence:
5'-CCGG-TCACTTCAGAAGTGGACACAA-CTCGAG-TTGTGTCCACTTCTGAAGTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCACTTCAGAAGTGGACACAACTCGAGTTGTGTCCACTTCTGAAGTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCACTTCAGAAGTGGACACAACTCGAGTTGTGTCCACTTCTGAAGTGA-3'

Other clones with same target sequence:

  1. TRCN0000055261