Construct: shRNA TRCN0000301396

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009532.4-1981s21c1
DNA Barcode:
CGGTGGATCTACAGTTGTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Xrcc1 (22594)
Gene Description:
X-ray repair complementing defective repair in Chinese hamster cells 1
Transcript:
RefSeq NM_009532.4 (NON-CURRENT)
Match location:
Position 1981 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000301396)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTGGATCTACAGTTGTAAT
Hairpin Sequence:
5'-CCGG-CGGTGGATCTACAGTTGTAAT-CTCGAG-ATTACAACTGTAGATCCACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTGGATCTACAGTTGTAATCTCGAGATTACAACTGTAGATCCACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTGGATCTACAGTTGTAATCTCGAGATTACAACTGTAGATCCACCG-3'

Other clones with same target sequence:

  1. TRCN0000077238