Construct: shRNA TRCN0000301415

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173396.2-385s21c1
DNA Barcode:
GCAGATATGTAACTGGTTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tgif2 (228839)
Gene Description:
TGFB-induced factor homeobox 2
Transcript:
RefSeq NM_173396.2 (NON-CURRENT)
Match location:
Position 385 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000301415)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATATGTAACTGGTTCAT
Hairpin Sequence:
5'-CCGG-GCAGATATGTAACTGGTTCAT-CTCGAG-ATGAACCAGTTACATATCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATATGTAACTGGTTCATCTCGAGATGAACCAGTTACATATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATATGTAACTGGTTCATCTCGAGATGAACCAGTTACATATCTGC-3'

Other clones with same target sequence:

  1. TRCN0000075524