Construct: shRNA TRCN0000301427

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017380.2-924s21c1
DNA Barcode:
CGACCATGAGTATCAAGTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sept9 (53860)
Gene Description:
septin 9
Transcript:
RefSeq NM_017380.2 (CURRENT)
Match location:
Position 924 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000301427)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACCATGAGTATCAAGTCAA
Hairpin Sequence:
5'-CCGG-CGACCATGAGTATCAAGTCAA-CTCGAG-TTGACTTGATACTCATGGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACCATGAGTATCAAGTCAACTCGAGTTGACTTGATACTCATGGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACCATGAGTATCAAGTCAACTCGAGTTGACTTGATACTCATGGTCG-3'

Other clones with same target sequence:

  1. TRCN0000077407