Construct: shRNA TRCN0000301430

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027288.3-349s21c1
DNA Barcode:
GCAGAAGGTAAAGTTGATCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Manba (110173)
Gene Description:
mannosidase, beta A, lysosomal
Transcript:
RefSeq NM_027288.3 (CURRENT)
Match location:
Position 349 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000301430)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAAGGTAAAGTTGATCTT
Hairpin Sequence:
5'-CCGG-GCAGAAGGTAAAGTTGATCTT-CTCGAG-AAGATCAACTTTACCTTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAAGGTAAAGTTGATCTTCTCGAGAAGATCAACTTTACCTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAAGGTAAAGTTGATCTTCTCGAGAAGATCAACTTTACCTTCTGC-3'

Other clones with same target sequence:

  1. TRCN0000077410