Construct: shRNA TRCN0000301452

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007859.4-701s21c1
DNA Barcode:
GCAGTACAATGGCAGCTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dffb (13368)
Gene Description:
DNA fragmentation factor, beta subunit
Transcript:
RefSeq NM_007859.4 (CURRENT)
Match location:
Position 701 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000301452)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTACAATGGCAGCTATTT
Hairpin Sequence:
5'-CCGG-GCAGTACAATGGCAGCTATTT-CTCGAG-AAATAGCTGCCATTGTACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTACAATGGCAGCTATTTCTCGAGAAATAGCTGCCATTGTACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTACAATGGCAGCTATTTCTCGAGAAATAGCTGCCATTGTACTGC-3'

Other clones with same target sequence:

  1. TRCN0000077214