Construct: shRNA TRCN0000301498

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007557.2-1448s21c1
DNA Barcode:
CGACGACAGCTCTAATGTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bmp7 (12162)
Gene Description:
bone morphogenetic protein 7
Transcript:
RefSeq NM_007557.2 (NON-CURRENT)
Match location:
Position 1448 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000301498)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACGACAGCTCTAATGTCAT
Hairpin Sequence:
5'-CCGG-CGACGACAGCTCTAATGTCAT-CTCGAG-ATGACATTAGAGCTGTCGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACGACAGCTCTAATGTCATCTCGAGATGACATTAGAGCTGTCGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACGACAGCTCTAATGTCATCTCGAGATGACATTAGAGCTGTCGTCG-3'

Other clones with same target sequence:

  1. TRCN0000065709