Construct: shRNA TRCN0000301517

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008707.3-278s21c1
DNA Barcode:
CAGGAAATACAGAAGGCCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nmt1 (18107)
Gene Description:
N-myristoyltransferase 1
Transcript:
RefSeq NM_008707.3 (CURRENT)
Match location:
Position 278 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000301517)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGAAATACAGAAGGCCATT
Hairpin Sequence:
5'-CCGG-CAGGAAATACAGAAGGCCATT-CTCGAG-AATGGCCTTCTGTATTTCCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGAAATACAGAAGGCCATTCTCGAGAATGGCCTTCTGTATTTCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGAAATACAGAAGGCCATTCTCGAGAATGGCCTTCTGTATTTCCTG-3'

Other clones with same target sequence:

  1. TRCN0000055167