Construct: shRNA TRCN0000301627

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009628.2-2775s21c1
DNA Barcode:
GATTCTTATGAGGCTAGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adnp (11538)
Gene Description:
activity-dependent neuroprotective protein
Transcript:
RefSeq NM_009628.2 (NON-CURRENT)
Match location:
Position 2775 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000301627)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTCTTATGAGGCTAGGAAA
Hairpin Sequence:
5'-CCGG-GATTCTTATGAGGCTAGGAAA-CTCGAG-TTTCCTAGCCTCATAAGAATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTCTTATGAGGCTAGGAAACTCGAGTTTCCTAGCCTCATAAGAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTCTTATGAGGCTAGGAAACTCGAGTTTCCTAGCCTCATAAGAATC-3'

Other clones with same target sequence:

  1. TRCN0000081671