Construct: shRNA TRCN0000301658

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133828.2-1324s21c1
DNA Barcode:
GCCTGAAAGCAACTACAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Creb1 (12912)
Gene Description:
cAMP responsive element binding protein 1
Transcript:
RefSeq NM_133828.2 (CURRENT)
Match location:
Position 1324 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000301658)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGAAAGCAACTACAGAAT
Hairpin Sequence:
5'-CCGG-GCCTGAAAGCAACTACAGAAT-CTCGAG-ATTCTGTAGTTGCTTTCAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGAAAGCAACTACAGAATCTCGAGATTCTGTAGTTGCTTTCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGAAAGCAACTACAGAATCTCGAGATTCTGTAGTTGCTTTCAGGC-3'

Other clones with same target sequence:

  1. TRCN0000096629
  2. TRCN0000356046