Construct: shRNA TRCN0000301713

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026148.2-206s21c1
DNA Barcode:
GAGAAGATCGTGAACAGTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lims1 (110829)
Gene Description:
LIM and senescent cell antigen-like domains 1
Transcript:
RefSeq NM_026148.2 (NON-CURRENT)
Match location:
Position 206 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000301713)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAAGATCGTGAACAGTAAT
Hairpin Sequence:
5'-CCGG-GAGAAGATCGTGAACAGTAAT-CTCGAG-ATTACTGTTCACGATCTTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAAGATCGTGAACAGTAATCTCGAGATTACTGTTCACGATCTTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAAGATCGTGAACAGTAATCTCGAGATTACTGTTCACGATCTTCTC-3'

Other clones with same target sequence:

  1. TRCN0000071465