Construct: shRNA TRCN0000301827

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153198.1-708s21c1
DNA Barcode:
GCTTCCATAAGGAAAGCAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hbp1 (73389)
Gene Description:
high mobility group box transcription factor 1
Transcript:
RefSeq NM_153198.1 (NON-CURRENT)
Match location:
Position 708 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000301827)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCCATAAGGAAAGCAATA
Hairpin Sequence:
5'-CCGG-GCTTCCATAAGGAAAGCAATA-CTCGAG-TATTGCTTTCCTTATGGAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCCATAAGGAAAGCAATACTCGAGTATTGCTTTCCTTATGGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCCATAAGGAAAGCAATACTCGAGTATTGCTTTCCTTATGGAAGC-3'

Other clones with same target sequence:

  1. TRCN0000084636