Construct: shRNA TRCN0000301910

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146222.2-835s21c1
DNA Barcode:
CGAGATACCATGCAGAATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Msantd2 (235184)
Gene Description:
Myb/SANT-like DNA-binding domain containing 2
Transcript:
RefSeq NM_146222.2 (CURRENT)
Match location:
Position 835 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000301910)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGATACCATGCAGAATATT
Hairpin Sequence:
5'-CCGG-CGAGATACCATGCAGAATATT-CTCGAG-AATATTCTGCATGGTATCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGATACCATGCAGAATATTCTCGAGAATATTCTGCATGGTATCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGATACCATGCAGAATATTCTCGAGAATATTCTGCATGGTATCTCG-3'

Other clones with same target sequence:

  1. TRCN0000085211