Construct: shRNA TRCN0000301911

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146222.2-746s21c1
DNA Barcode:
GCTATCCAACAGATCAGGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Msantd2 (235184)
Gene Description:
Myb/SANT-like DNA-binding domain containing 2
Transcript:
RefSeq NM_146222.2 (CURRENT)
Match location:
Position 746 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000301911)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATCCAACAGATCAGGAAT
Hairpin Sequence:
5'-CCGG-GCTATCCAACAGATCAGGAAT-CTCGAG-ATTCCTGATCTGTTGGATAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATCCAACAGATCAGGAATCTCGAGATTCCTGATCTGTTGGATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATCCAACAGATCAGGAATCTCGAGATTCCTGATCTGTTGGATAGC-3'

Other clones with same target sequence:

  1. TRCN0000085210