Construct: shRNA TRCN0000301919

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011566.3-1540s21c1
DNA Barcode:
CCACGTCTACAAGCTTGTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tead3 (21678)
Gene Description:
TEA domain family member 3
Transcript:
RefSeq NM_011566.3 (NON-CURRENT)
Match location:
Position 1540 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000301919)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACGTCTACAAGCTTGTCAA
Hairpin Sequence:
5'-CCGG-CCACGTCTACAAGCTTGTCAA-CTCGAG-TTGACAAGCTTGTAGACGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACGTCTACAAGCTTGTCAACTCGAGTTGACAAGCTTGTAGACGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACGTCTACAAGCTTGTCAACTCGAGTTGACAAGCTTGTAGACGTGG-3'

Other clones with same target sequence:

  1. TRCN0000085949