Construct: shRNA TRCN0000302046

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022011.4-1484s21c1
DNA Barcode:
GAAAGCACTTTGAAAGAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gtf2h2 (23894)
Gene Description:
general transcription factor II H, polypeptide 2
Transcript:
RefSeq NM_022011.4 (CURRENT)
Match location:
Position 1484 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000302046)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGCACTTTGAAAGAAGAT
Hairpin Sequence:
5'-CCGG-GAAAGCACTTTGAAAGAAGAT-CTCGAG-ATCTTCTTTCAAAGTGCTTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGCACTTTGAAAGAAGATCTCGAGATCTTCTTTCAAAGTGCTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGCACTTTGAAAGAAGATCTCGAGATCTTCTTTCAAAGTGCTTTC-3'

Other clones with same target sequence:

  1. TRCN0000085828