Construct: shRNA TRCN0000302052

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028260.2-667s21c1
DNA Barcode:
CCTTTCAGTGACTTCGGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Immp1l (66541)
Gene Description:
IMP1 inner mitochondrial membrane peptidase-like (S. cerevisiae)
Transcript:
RefSeq NM_028260.2 (CURRENT)
Match location:
Position 667 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000302052)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTCAGTGACTTCGGATTT
Hairpin Sequence:
5'-CCGG-CCTTTCAGTGACTTCGGATTT-CTCGAG-AAATCCGAAGTCACTGAAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTCAGTGACTTCGGATTTCTCGAGAAATCCGAAGTCACTGAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTCAGTGACTTCGGATTTCTCGAGAAATCCGAAGTCACTGAAAGG-3'

Other clones with same target sequence:

  1. TRCN0000222022