Construct: shRNA TRCN0000302060

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027882.3-2053s21c1
DNA Barcode:
CCGACATTGATCTCAAGTGCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cic (71722)
Gene Description:
capicua transcriptional repressor
Transcript:
RefSeq NM_027882.3 (NON-CURRENT)
Match location:
Position 2053 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000302060)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGACATTGATCTCAAGTGCA
Hairpin Sequence:
5'-CCGG-CCGACATTGATCTCAAGTGCA-CTCGAG-TGCACTTGAGATCAATGTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGACATTGATCTCAAGTGCACTCGAGTGCACTTGAGATCAATGTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGACATTGATCTCAAGTGCACTCGAGTGCACTTGAGATCAATGTCGG-3'

Other clones with same target sequence:

  1. TRCN0000082012