Construct: shRNA TRCN0000302073

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009530.2-1412s21c1
DNA Barcode:
GCTATGAAGTTATGTCAACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atrx (22589)
Gene Description:
ATRX, chromatin remodeler
Transcript:
RefSeq NM_009530.2 (CURRENT)
Match location:
Position 1412 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000302073)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATGAAGTTATGTCAACTT
Hairpin Sequence:
5'-CCGG-GCTATGAAGTTATGTCAACTT-CTCGAG-AAGTTGACATAACTTCATAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATGAAGTTATGTCAACTTCTCGAGAAGTTGACATAACTTCATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATGAAGTTATGTCAACTTCTCGAGAAGTTGACATAACTTCATAGC-3'

Other clones with same target sequence:

  1. TRCN0000081912