Construct: shRNA TRCN0000302162

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023773.2-2634s21c1
DNA Barcode:
GCCAAGGTTAAGTTGCTAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mphosph8 (75339)
Gene Description:
M-phase phosphoprotein 8
Transcript:
RefSeq NM_023773.2 (CURRENT)
Match location:
Position 2634 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000302162)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGGTTAAGTTGCTAATA
Hairpin Sequence:
5'-CCGG-GCCAAGGTTAAGTTGCTAATA-CTCGAG-TATTAGCAACTTAACCTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGGTTAAGTTGCTAATACTCGAGTATTAGCAACTTAACCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGGTTAAGTTGCTAATACTCGAGTATTAGCAACTTAACCTTGGC-3'

Other clones with same target sequence:

  1. TRCN0000085440