Construct: shRNA TRCN0000302193

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011420.2-374s21c1
DNA Barcode:
CCATTGACTTTAAGAGAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smn1 (20595)
Gene Description:
survival motor neuron 1
Transcript:
RefSeq NM_011420.2 (CURRENT)
Match location:
Position 374 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000302193)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATTGACTTTAAGAGAGAAA
Hairpin Sequence:
5'-CCGG-CCATTGACTTTAAGAGAGAAA-CTCGAG-TTTCTCTCTTAAAGTCAATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATTGACTTTAAGAGAGAAACTCGAGTTTCTCTCTTAAAGTCAATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATTGACTTTAAGAGAGAAACTCGAGTTTCTCTCTTAAAGTCAATGG-3'

Other clones with same target sequence:

  1. TRCN0000072020