Construct: shRNA TRCN0000302217

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019709.3-3378s21c1
DNA Barcode:
GCACCCAGTAATCTTTGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mbtps1 (56453)
Gene Description:
membrane-bound transcription factor peptidase, site 1
Transcript:
RefSeq NM_019709.3 (NON-CURRENT)
Match location:
Position 3378 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000302217)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCCAGTAATCTTTGGAAA
Hairpin Sequence:
5'-CCGG-GCACCCAGTAATCTTTGGAAA-CTCGAG-TTTCCAAAGATTACTGGGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCCAGTAATCTTTGGAAACTCGAGTTTCCAAAGATTACTGGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCCAGTAATCTTTGGAAACTCGAGTTTCCAAAGATTACTGGGTGC-3'

Other clones with same target sequence:

  1. TRCN0000032815