Construct: shRNA TRCN0000302225

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153787.2-1544s21c1
DNA Barcode:
CCCAAGTACAAGTCCAAAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bclaf1 (72567)
Gene Description:
BCL2-associated transcription factor 1
Transcript:
RefSeq NM_153787.2 (CURRENT)
Match location:
Position 1544 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000302225)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAGTACAAGTCCAAAGTT
Hairpin Sequence:
5'-CCGG-CCCAAGTACAAGTCCAAAGTT-CTCGAG-AACTTTGGACTTGTACTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAGTACAAGTCCAAAGTTCTCGAGAACTTTGGACTTGTACTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAGTACAAGTCCAAAGTTCTCGAGAACTTTGGACTTGTACTTGGG-3'

Other clones with same target sequence:

  1. TRCN0000084417