Construct: shRNA TRCN0000302271

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053170.2-2918s21c1
DNA Barcode:
GCTCATAGTTTCAAGTCTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trim33 (94093)
Gene Description:
tripartite motif-containing 33
Transcript:
RefSeq NM_053170.2 (NON-CURRENT)
Match location:
Position 2918 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000302271)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCATAGTTTCAAGTCTGAT
Hairpin Sequence:
5'-CCGG-GCTCATAGTTTCAAGTCTGAT-CTCGAG-ATCAGACTTGAAACTATGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCATAGTTTCAAGTCTGATCTCGAGATCAGACTTGAAACTATGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCATAGTTTCAAGTCTGATCTCGAGATCAGACTTGAAACTATGAGC-3'

Other clones with same target sequence:

  1. TRCN0000039531