Construct: shRNA TRCN0000302282

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023230.2-230s21c1
DNA Barcode:
CCGAATATACAGCCTTAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ube2v1 (66589)
Gene Description:
ubiquitin-conjugating enzyme E2 variant 1
Transcript:
RefSeq NM_023230.2 (CURRENT)
Match location:
Position 230 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000302282)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAATATACAGCCTTAAGAT
Hairpin Sequence:
5'-CCGG-CCGAATATACAGCCTTAAGAT-CTCGAG-ATCTTAAGGCTGTATATTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAATATACAGCCTTAAGATCTCGAGATCTTAAGGCTGTATATTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAATATACAGCCTTAAGATCTCGAGATCTTAAGGCTGTATATTCGG-3'

Other clones with same target sequence:

  1. TRCN0000039512