Construct: shRNA TRCN0000302320
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_013787.2-340s21c2
- DNA Barcode:
- GCAAGACTTCTGAACTGCTAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Skp2 (27401)
- Gene Description:
- S-phase kinase-associated protein 2 (p45)
- Transcript:
- RefSeq NM_013787.2 (NON-CURRENT)
- Match location:
- Position 340 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000302320)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCAAGACTTCTGAACTGCTAT
- Hairpin Sequence:
-
5'-CCGG-GCAAGACTTCTGAACTGCTAT-CTCGAG-ATAGCAGTTCAGAAGTCTTGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCAAGACTTCTGAACTGCTATCTCGAGATAGCAGTTCAGAAGTCTTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCAAGACTTCTGAACTGCTATCTCGAGATAGCAGTTCAGAAGTCTTGC-3'
Other clones with same target sequence:
-
TRCN0000088761
