Construct: shRNA TRCN0000302323

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001077495.1-2508s21c1
DNA Barcode:
CAACCGAAACAAAGCGGAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pik3r1 (18708)
Gene Description:
phosphoinositide-3-kinase regulatory subunit 1
Transcript:
RefSeq NM_001077495.1 (NON-CURRENT)
Match location:
Position 2508 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000302323)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACCGAAACAAAGCGGAGAA
Hairpin Sequence:
5'-CCGG-CAACCGAAACAAAGCGGAGAA-CTCGAG-TTCTCCGCTTTGTTTCGGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACCGAAACAAAGCGGAGAACTCGAGTTCTCCGCTTTGTTTCGGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACCGAAACAAAGCGGAGAACTCGAGTTCTCCGCTTTGTTTCGGTTG-3'

Other clones with same target sequence:

  1. TRCN0000068196