Construct: shRNA TRCN0000302340

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011666.2-825s21c1
DNA Barcode:
CCCAGAACACTGTATCGAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Uba3 (22200)
Gene Description:
ubiquitin-like modifier activating enzyme 3
Transcript:
RefSeq NM_011666.2 (NON-CURRENT)
Match location:
Position 825 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000302340)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGAACACTGTATCGAGTA
Hairpin Sequence:
5'-CCGG-CCCAGAACACTGTATCGAGTA-CTCGAG-TACTCGATACAGTGTTCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGAACACTGTATCGAGTACTCGAGTACTCGATACAGTGTTCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGAACACTGTATCGAGTACTCGAGTACTCGATACAGTGTTCTGGG-3'

Other clones with same target sequence:

  1. TRCN0000039503