Construct: shRNA TRCN0000302365

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008281.3-539s21c1
DNA Barcode:
CAACGGCACATCGGGCTTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hpn (15451)
Gene Description:
hepsin
Transcript:
RefSeq NM_008281.3 (NON-CURRENT)
Match location:
Position 539 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000302365)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACGGCACATCGGGCTTCTT
Hairpin Sequence:
5'-CCGG-CAACGGCACATCGGGCTTCTT-CTCGAG-AAGAAGCCCGATGTGCCGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACGGCACATCGGGCTTCTTCTCGAGAAGAAGCCCGATGTGCCGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACGGCACATCGGGCTTCTTCTCGAGAAGAAGCCCGATGTGCCGTTG-3'

Other clones with same target sequence:

  1. TRCN0000054790