Construct: shRNA TRCN0000302403

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178775.3-1846s21c1
DNA Barcode:
CCGTCCCAGTTATCTCATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rps6kc1 (320119)
Gene Description:
ribosomal protein S6 kinase polypeptide 1
Transcript:
RefSeq NM_178775.3 (NON-CURRENT)
Match location:
Position 1846 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000302403)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTCCCAGTTATCTCATTTA
Hairpin Sequence:
5'-CCGG-CCGTCCCAGTTATCTCATTTA-CTCGAG-TAAATGAGATAACTGGGACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTCCCAGTTATCTCATTTACTCGAGTAAATGAGATAACTGGGACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTCCCAGTTATCTCATTTACTCGAGTAAATGAGATAACTGGGACGG-3'

Other clones with same target sequence:

  1. TRCN0000078901