Construct: shRNA TRCN0000302420

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028876.2-679s21c1
DNA Barcode:
GCCACATACAAACTCTACTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmed5 (73130)
Gene Description:
transmembrane p24 trafficking protein 5
Transcript:
RefSeq NM_028876.2 (CURRENT)
Match location:
Position 679 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000302420)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACATACAAACTCTACTTA
Hairpin Sequence:
5'-CCGG-GCCACATACAAACTCTACTTA-CTCGAG-TAAGTAGAGTTTGTATGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACATACAAACTCTACTTACTCGAGTAAGTAGAGTTTGTATGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACATACAAACTCTACTTACTCGAGTAAGTAGAGTTTGTATGTGGC-3'

Other clones with same target sequence:

  1. TRCN0000112351