Construct: shRNA TRCN0000302443

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029945.2-1743s21c1
DNA Barcode:
CCCAATGTTTGGTCCTGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smpd4 (77626)
Gene Description:
sphingomyelin phosphodiesterase 4
Transcript:
RefSeq NM_029945.2 (NON-CURRENT)
Match location:
Position 1743 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000302443)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAATGTTTGGTCCTGAAAT
Hairpin Sequence:
5'-CCGG-CCCAATGTTTGGTCCTGAAAT-CTCGAG-ATTTCAGGACCAAACATTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAATGTTTGGTCCTGAAATCTCGAGATTTCAGGACCAAACATTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAATGTTTGGTCCTGAAATCTCGAGATTTCAGGACCAAACATTGGG-3'

Other clones with same target sequence:

  1. TRCN0000124166