Construct: shRNA TRCN0000302466

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025864.3-1260s21c1
DNA Barcode:
CCAGTGAATAACCTGAAACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pacc1 (66950)
Gene Description:
proton activated chloride channel 1
Transcript:
RefSeq NM_025864.3 (NON-CURRENT)
Match location:
Position 1260 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000302466)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTGAATAACCTGAAACTT
Hairpin Sequence:
5'-CCGG-CCAGTGAATAACCTGAAACTT-CTCGAG-AAGTTTCAGGTTATTCACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTGAATAACCTGAAACTTCTCGAGAAGTTTCAGGTTATTCACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTGAATAACCTGAAACTTCTCGAGAAGTTTCAGGTTATTCACTGG-3'

Other clones with same target sequence:

  1. TRCN0000125299