Construct: shRNA TRCN0000302509

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016926.1-3420s21c1
DNA Barcode:
GCGACCTTTGTTCTTAGGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sart3 (53890)
Gene Description:
squamous cell carcinoma antigen recognized by T cells 3
Transcript:
RefSeq NM_016926.1 (CURRENT)
Match location:
Position 3420 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000302509)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGACCTTTGTTCTTAGGAAT
Hairpin Sequence:
5'-CCGG-GCGACCTTTGTTCTTAGGAAT-CTCGAG-ATTCCTAAGAACAAAGGTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGACCTTTGTTCTTAGGAATCTCGAGATTCCTAAGAACAAAGGTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGACCTTTGTTCTTAGGAATCTCGAGATTCCTAAGAACAAAGGTCGC-3'

Other clones with same target sequence:

  1. TRCN0000124204