Construct: shRNA TRCN0000302792

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010863.2-2324s21c1
DNA Barcode:
CCACTCTCATTCAGAAGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Myo1b (17912)
Gene Description:
myosin IB
Transcript:
RefSeq NM_010863.2 (NON-CURRENT)
Match location:
Position 2324 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000302792)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTCTCATTCAGAAGATTT
Hairpin Sequence:
5'-CCGG-CCACTCTCATTCAGAAGATTT-CTCGAG-AAATCTTCTGAATGAGAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTCTCATTCAGAAGATTTCTCGAGAAATCTTCTGAATGAGAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTCTCATTCAGAAGATTTCTCGAGAAATCTTCTGAATGAGAGTGG-3'

Other clones with same target sequence:

  1. TRCN0000100866