Construct: shRNA TRCN0000303025

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182650.3-989s21c1
DNA Barcode:
GCCTTCTAACTATGGTCCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hnrnpa2b1 (53379)
Gene Description:
heterogeneous nuclear ribonucleoprotein A2/B1
Transcript:
RefSeq NM_182650.3 (NON-CURRENT)
Match location:
Position 989 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000303025)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTCTAACTATGGTCCAAT
Hairpin Sequence:
5'-CCGG-GCCTTCTAACTATGGTCCAAT-CTCGAG-ATTGGACCATAGTTAGAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTCTAACTATGGTCCAATCTCGAGATTGGACCATAGTTAGAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTCTAACTATGGTCCAATCTCGAGATTGGACCATAGTTAGAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000071142