Construct: shRNA TRCN0000303055

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145575.3-1643s21c1
DNA Barcode:
GCCGCATTAACGAATGGCTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cald1 (109624)
Gene Description:
caldesmon 1
Transcript:
RefSeq NM_145575.3 (CURRENT)
Match location:
Position 1643 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000303055)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGCATTAACGAATGGCTAA
Hairpin Sequence:
5'-CCGG-GCCGCATTAACGAATGGCTAA-CTCGAG-TTAGCCATTCGTTAATGCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGCATTAACGAATGGCTAACTCGAGTTAGCCATTCGTTAATGCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGCATTAACGAATGGCTAACTCGAGTTAGCCATTCGTTAATGCGGC-3'

Other clones with same target sequence:

  1. TRCN0000108681