Construct: shRNA TRCN0000303077

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017398.3-2461s21c1
DNA Barcode:
GCCCTAATCCAGAATCTTGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Diaph2 (54004)
Gene Description:
diaphanous related formin 2
Transcript:
RefSeq NM_017398.3 (NON-CURRENT)
Match location:
Position 2461 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000303077)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTAATCCAGAATCTTGTA
Hairpin Sequence:
5'-CCGG-GCCCTAATCCAGAATCTTGTA-CTCGAG-TACAAGATTCTGGATTAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTAATCCAGAATCTTGTACTCGAGTACAAGATTCTGGATTAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTAATCCAGAATCTTGTACTCGAGTACAAGATTCTGGATTAGGGC-3'

Other clones with same target sequence:

  1. TRCN0000108782