Construct: shRNA TRCN0000303108

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028487.4-2390s21c1
DNA Barcode:
GCAATACTACTCACCAAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpbp1 (73274)
Gene Description:
GC-rich promoter binding protein 1
Transcript:
RefSeq NM_028487.4 (CURRENT)
Match location:
Position 2390 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000303108)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAATACTACTCACCAAGAAA
Hairpin Sequence:
5'-CCGG-GCAATACTACTCACCAAGAAA-CTCGAG-TTTCTTGGTGAGTAGTATTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAATACTACTCACCAAGAAACTCGAGTTTCTTGGTGAGTAGTATTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAATACTACTCACCAAGAAACTCGAGTTTCTTGGTGAGTAGTATTGC-3'

Other clones with same target sequence:

  1. TRCN0000071137