Construct: shRNA TRCN0000303191

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017366.2-149s21c1
DNA Barcode:
GCGATCTACTACTGTGCTTCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Acadvl (11370)
Gene Description:
acyl-Coenzyme A dehydrogenase, very long chain
Transcript:
RefSeq NM_017366.2 (NON-CURRENT)
Match location:
Position 149 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000303191)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGATCTACTACTGTGCTTCA
Hairpin Sequence:
5'-CCGG-GCGATCTACTACTGTGCTTCA-CTCGAG-TGAAGCACAGTAGTAGATCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGATCTACTACTGTGCTTCACTCGAGTGAAGCACAGTAGTAGATCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGATCTACTACTGTGCTTCACTCGAGTGAAGCACAGTAGTAGATCGC-3'

Other clones with same target sequence:

  1. TRCN0000041330