Construct: shRNA TRCN0000303245

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029878.2-1538s21c1
DNA Barcode:
GCTGCTGTATTTGACCGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tbcd (108903)
Gene Description:
tubulin-specific chaperone d
Transcript:
RefSeq NM_029878.2 (NON-CURRENT)
Match location:
Position 1538 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000303245)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTGTATTTGACCGAAAT
Hairpin Sequence:
5'-CCGG-GCTGCTGTATTTGACCGAAAT-CTCGAG-ATTTCGGTCAAATACAGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTGTATTTGACCGAAATCTCGAGATTTCGGTCAAATACAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTGTATTTGACCGAAATCTCGAGATTTCGGTCAAATACAGCAGC-3'

Other clones with same target sequence:

  1. TRCN0000091145