Construct: shRNA TRCN0000303267

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178878.2-499s21c1
DNA Barcode:
GCCAATACAGAATAGCAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hadha (97212)
Gene Description:
hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
Transcript:
RefSeq NM_178878.2 (CURRENT)
Match location:
Position 499 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000303267)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAATACAGAATAGCAACAA
Hairpin Sequence:
5'-CCGG-GCCAATACAGAATAGCAACAA-CTCGAG-TTGTTGCTATTCTGTATTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAATACAGAATAGCAACAACTCGAGTTGTTGCTATTCTGTATTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAATACAGAATAGCAACAACTCGAGTTGTTGCTATTCTGTATTGGC-3'

Other clones with same target sequence:

  1. TRCN0000041351