Construct: shRNA TRCN0000303279

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003680.3-1136s21c1
DNA Barcode:
GTTCATCAAAGGCACTGATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
YARS1 (8565)
Gene Description:
tyrosyl-tRNA synthetase 1
Transcript:
RefSeq NM_003680.3 (CURRENT)
Match location:
Position 1136 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000303279)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCATCAAAGGCACTGATTA
Hairpin Sequence:
5'-CCGG-GTTCATCAAAGGCACTGATTA-CTCGAG-TAATCAGTGCCTTTGATGAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCATCAAAGGCACTGATTACTCGAGTAATCAGTGCCTTTGATGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCATCAAAGGCACTGATTACTCGAGTAATCAGTGCCTTTGATGAAC-3'

Other clones with same target sequence:

(none)