Construct: shRNA TRCN0000303291

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004663.3-460s21c1
DNA Barcode:
ATCATGCTGATAGTAACATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAB11A (8766)
Gene Description:
RAB11A, member RAS oncogene family
Transcript:
RefSeq NM_004663.3 (NON-CURRENT)
Match location:
Position 460 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000303291)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCATGCTGATAGTAACATTG
Hairpin Sequence:
5'-CCGG-ATCATGCTGATAGTAACATTG-CTCGAG-CAATGTTACTATCAGCATGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCATGCTGATAGTAACATTGCTCGAGCAATGTTACTATCAGCATGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCATGCTGATAGTAACATTGCTCGAGCAATGTTACTATCAGCATGAT-3'

Other clones with same target sequence:

(none)