Construct: shRNA TRCN0000303297

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015271.3-2252s21c1
DNA Barcode:
TTTGATGGGAGTGGATCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRIM2 (23321)
Gene Description:
tripartite motif containing 2
Transcript:
RefSeq NM_015271.3 (NON-CURRENT)
Match location:
Position 2252 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000303297)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTGATGGGAGTGGATCATTT
Hairpin Sequence:
5'-CCGG-TTTGATGGGAGTGGATCATTT-CTCGAG-AAATGATCCACTCCCATCAAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTGATGGGAGTGGATCATTTCTCGAGAAATGATCCACTCCCATCAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTGATGGGAGTGGATCATTTCTCGAGAAATGATCCACTCCCATCAAA-3'

Other clones with same target sequence:

(none)