Construct: shRNA TRCN0000303461

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000503.4-748s21c1
DNA Barcode:
TCCCAATGGCACCGAAGTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EYA1 (2138)
Gene Description:
EYA transcriptional coactivator and phosphatase 1
Transcript:
RefSeq NM_000503.4 (NON-CURRENT)
Match location:
Position 748 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000303461)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCCAATGGCACCGAAGTTAA
Hairpin Sequence:
5'-CCGG-TCCCAATGGCACCGAAGTTAA-CTCGAG-TTAACTTCGGTGCCATTGGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCCAATGGCACCGAAGTTAACTCGAGTTAACTTCGGTGCCATTGGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCCAATGGCACCGAAGTTAACTCGAGTTAACTTCGGTGCCATTGGGA-3'

Other clones with same target sequence:

  1. TRCN0000366189