Construct: shRNA TRCN0000303469

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012326.2-428s21c1
DNA Barcode:
CTGTAGAGAAATTAGTGAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPRE3 (22924)
Gene Description:
microtubule associated protein RP/EB family member 3
Transcript:
RefSeq NM_012326.2 (NON-CURRENT)
Match location:
Position 428 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000303469)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTAGAGAAATTAGTGAAAG
Hairpin Sequence:
5'-CCGG-CTGTAGAGAAATTAGTGAAAG-CTCGAG-CTTTCACTAATTTCTCTACAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTAGAGAAATTAGTGAAAGCTCGAGCTTTCACTAATTTCTCTACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTAGAGAAATTAGTGAAAGCTCGAGCTTTCACTAATTTCTCTACAG-3'

Other clones with same target sequence:

(none)